Table 1.
Primer Target | Oligonucleotide Sequence (5′-3′) | Amplicon Size (bp) | Target Gene | TM °C | References |
---|---|---|---|---|---|
toxA-F | TGCTGCACTACTCCATGGTC | 190 | toxA | 53.8 | (24) |
toxA-R | ATCGGTACCAGCCAGTTCAG | 53.8 | |||
exoU- F | GCTAAGGCTTGGCGGAATA | 204 | exoU | 51.1 | (10) |
exoU-R | AGATCACACCCAGCGGTAAC | 53.8 | |||
exoS- F | ATGTCAGCGGGATATCGAAC | 230 | exoS | 51.1 | (10) |
exoS-R | CAGGCGTACATCCTGTTCCT | 53.8 |