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. 2019 Mar 15;4(1):ysz008. doi: 10.1093/synbio/ysz008

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© The Author(s) 2019. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — Effect of IS-knockdown on fluorescence of individual cultures. (A) Maximum fluorescence intensities (AU) of the individual cultures in the presence of either an IS-silencing plasmid (pCRIS) or a nontargeting control plasmid (pCRISPathBrick), after 7 days (60 generations) of continuous propagation (T₆₀). (B) Analysis of the mutagenic spectrum within the km-gfp cassette of pBDP_Km_GFP5 from individual cell-populations (n = 48) at T₆₀. Arrows mark PCR products displaying an increased length due to IS transposition into km-gfp.