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. 2019 Apr 13;7:60. doi: 10.1186/s40168-019-0674-x

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — a Representative scanning electron micrographs of ureteral stent encrustations and biofilms. Numbers refer to sample IDs. Based on NGS and cultivation, bacteria visible likely include L. jensenii (ST55), G. vaginalis (ST22), S. anginosus or A. tetradius (ST08), Staphylococcus (ST45), S. epidermidis (ST85) and Corynebacterium (ST30). Further samples included structures with size and morphology of fungal cells or blood cells (ST05, ST51 and ST80). Scale bars = 5 μm. b Confocal laser scanning micrographs of SYBR Green-stained ureteral stent encrustations, shown as maximum intensity projection. Fluorescence was associated with a layered structure. Colour allocation—grey, reflection; green, SYBR Green. Scale bars = 50 μm