Skip to main content
. 2019 Mar 31;2019:7564207. doi: 10.1155/2019/7564207

Figure 4.

Figure 4

Kaempferol protects mitochondrial membrane integrity. (a-c) Mesangial cells were exposed to D-ribose for 48 h with or without the treatment of kaempferol (1, 2, and 5 μM). (a) JC-1 staining was used to investigate mitochondrial depolarization. Cells with depolarized mitochondria were detected by green fluorescence and quantified as the percentage of total cell, bar = 100 μm. (b) Western blot was used to examine the expression of Bax and Bcl-2 protein. (c) RT-PCR was used to investigate the mRNA level of Bax and Bcl-2. Data were expressed as mean ± SEM from independent experiments. Comparisons between multiple groups were analyzed by one-way ANOVA followed by the Turkey posttest. ##P < 0.01, relative to the control group; ∗P < 0.05 and ∗∗P < 0.01, relative to the D-ribose group.