Figure 2. N-glycan homogeneity can be achieved through post purification remodeling.

The predominant N-glycoforms found on hIgG1 Fc expressed from HEK293F cells can be remodeled with specific enzymes and substrates. The glycans are predominantly a complex-type biantennary N-glycan with varying levels of galactose and sialic acid incorporation (shown on the left side of the figure). NMR active isotopes can be incorporated using the glycosyltransferase enzymes noted above. Also note the paucimannose structure in the bottom right of the figure with two N-acetylglucosamine and three mannose residues (in this example the paucimannose N-glycan contains a core fucose residue).