Table 1. Primers and probes used to detect RSV, hMPV, and influenza virus.
| Virus | Primer/Probe | Sequence 5′–3′ | Target | Position | ||
|---|---|---|---|---|---|---|
| Assay 1 | RSV A and B | RSV_F3251 | GGCAAATATGGAAACATACGTGAA | M | 3251-3274 | (+) |
| RSV_R3334 | TCTTTTTCTAGGACATTGTAYTGAACAG | M | 3334-3361 | (-) | ||
| RSV_P3303 | FAM-CTGTGTATGTGGAGCCTTCGTGAAGCT-BHQ1 | M | 3303-3329 | (+) | ||
| hMPV A and B | hMPV_F3604 | CAARTGYGACATTGCTGAYCTRAA | F | 3604-3628 | (+) | |
| hMPV_R3683 | ACTGCCGCACAACATTTAGRAA | F | 3683-3662 | (-) | ||
| HMPV_P3630 | JOE-TGGCYGTYAGCTTCAGTCARTTC-BHQ1 | F | 3630-3643 | (+) | ||
| Assay 2a | Influenza A | FluA-M-F151 | CATGGARTGGCTAAAGACAAGACC | M | 151-175 | (+) |
| FluA-M-R276 | AGGGCATTTTGGACAAAKCGTCTA | M | 276-252 | (-) | ||
| FluA-M-P218 | FAM-ACGCTCACCGTGCCCAGT-BHQ1 | M | 218-235 | (+) | ||
| Influenza B | FluB-MF439 | CTCTGTGCTTTRTGCGARAAAC | M | 439-460 | (+) | |
| FluB-MR | CCTTCYCCATTCTTTTGACTTGC | M | 671-649 | (-) | ||
| FluB-P135 | Cy5-TCAGCAATGAACACAGCAA-BHQ3 | M | 541-559 | (+) | ||
| Influenza A/H1N1 | H1_F | ACTACTGGACTCTGCTKGAA | H1 | 750-769 | (+) | |
| H1_R | AAGCCTCTACTCAGTGCGAA | H1 | 846-827 | (-) | ||
| H1_P | FAM-TTGAGGCAAATGGAAATCTAATAGC-TAMRA | H1 | 789-813 | (+) | ||
| Influenza A/H3N2 | H3_F | TGCTACTGAGCTGGTTCAGAGT | H3 | 139-160 | (+) | |
| H3_R | AGGGTAACAGTTGCTGTRGGC | H3 | 322-302 | (-) | ||
| H3_P | HEX-AGATGCTCTATTGGGAGACC-BHQ1 | H3 | 226-245 | (+) | ||
| GAPDH | GAPDH-F85 | GTGAAGGTCGGAGTCAACGG | GAPDH | 85-104 | (+) | |
| GAPDH-R191 | TCAATGAAGGGGTCATTGATGG | GAPDH | 191-169 | (-) | ||
| GAPDH-P121 | HEX-CGCCTGGTCACCAGGGCTGC-BHQ1 | GAPDH | 121-140 | (+) |
Notes.
a
Previously described in Suwannakarn et al. (2008).
(+) and (-) denote sense and anti-sense strand, respectively.