Figure 2.

The effect of O-GlcNAcase inhibition on polysome association is consistent with sequencing analysis. Mice were administered TMG or PBS vehicle. A, summary of results from the sequencing analysis described in Fig. 1 for mRNAs encoding Nr6a1, Fam122a, Elmsan1, Mettl14, Nat8l, and Il1Rap. B–D, ribosomes were separated into either subpolysomal (SP) or polysomal (P) fractions via sucrose density gradient centrifugation. The distribution of mRNAs encoding Nr6a1, Fam122a, Elmsan1, Mettl14, Nat8l, and Il1Rap were assessed in subpolysomal or polysomal fractions via RT-PCR. Results are expressed as a relative percentage of the mRNA in the polysomal fraction and are representative of two independent experiments. Values are means ± S.E. for three replicates, assessing a pooled sample obtained from 10 retinas. *, p < 0.05 versus PBS vehicle.