Figure 5.

mGFP–hDAT dimerization at the plasma membrane is independent of PIP₂ levels. A, we enzymatically depleted PIP₂ at the plasma membrane via activation of PLCγ by incubating cells for 20 min with the direct PLCγ activator m-3M3FBS (25 μm). m-3M3FBS did not yield any effect on the oligomeric distribution (gray bars) compared with the untreated cells (white bars) (n > 80 cells per experimental condition). mGFP–hDAT surface densities were similar: 35 ± 4 μm² (white bars) and 33 ± 5 μm² (gray bars). B, the oligomeric distribution is plotted for various mGFP–hDAT surface density (n > 40 cells) following PIP₂ depletion via m-3M3FBS; mGFP–hDAT densities are ∼7 mGFP–hDAT/μm² for low surface density and ∼40 mGFP–hDAT/μm² for high surface density. Error bars show the mean S.E.