Figure 1.

GluA1/GluA2 heteromers are resistant to acute elevation PKC regardless of the presence of AKAP79. A, HEK 293 cells were transfected with GluA1/GluA2 with or without AKAP79-GFP. The catalytic fragment of PKC (PKM) was included in the pipette recording solution. Currents were evoked every 30 s in response to glutamate (1 mm) in the presence of cyclothiazide (100 μm). Shown is a summary time course for glutamate-evoked currents. Currents for each cell were normalized to their initial amplitude. Only cells with minimal rectification indicative of heteromers were included in the analysis. B, averaged I-V relationships for cells expressing GluA1/GluA2 with or without AKAP79-GFP obtained via ramp protocol at the beginning and end of each recording. Error bars, S.E.