Figure 4.

GluA1 basal phosphorylation is elevated by GluA2 co-expression and apparently uncoupled from AKAP79. HEK 293 cells were transfected with either GluA1 or GluA1/GluA2 with or without AKAP79-GFP. A (top), representative Western blotting (IB) for S831P and GluA1 for each of the indicated conditions. Bottom, summary graph showing the ratio of S831P/GluA1 for multiple experiments. Data for each experiment were normalized to those obtained for GluA1 alone. Circles, data from individual experiments. B, parallel analysis of Ser-845/GluA1 ratio is shown. *, p < 0.05; **, p < 0.01, compared with GluA1 alone. Error bars, S.E.