Figure 2.

The m¹G37 modification in ${\text{tRNA}}_{\text{CGG}}^{\text{Pro}}$ is important for binding to a cognate CCG codon. A, equilibrium binding of ${\text{ASL}}_{\text{CGG}}^{\text{Pro}}$ with (+m¹G37) or without (Δm¹G37) the modification to a programmed 70S containing an A-site CCG codon. B, schematic of the association and dissociation of tRNA from the ribosomal A site. C and D, k_on (C) and k_off (D) rates of ${\text{ASL}}_{\text{CGG}}^{\text{Pro}}$ with (+m¹G37) or without (Δm¹G37) the modification to the 70S containing a cognate CCG codon in the A site. Data are the mean ± S.E. (error bars) of at least five independent experiments.