Figure 1.

Iron treatment decreases total O-GlcNAcylation in 3T3-L1 adipocytes and down-regulates leptin. A, total protein O-GlcNAcylation as revealed by Western blotting using a pan-O-GlcNAc antibody in control (CTL) cells and cells treated with 100 μg/ml FAC. B, quantification of Western blots normalized to α-tubulin (n = 3 independent determinations). C, leptin (Ob) mRNA in cells treated with or without FAC (n = 6 independent determinations). *, p < 0.05 using by two-tailed Student's t test. The data represent means ± S.E.