Figure 1.

Folts model. (A) Basal blood flow in the carotid artery is significantly higher when mice are anesthetized with isoflurane and ket‐x than compared to pentobarbitone (n = 6). (B) Greater degree of blood flow restriction is necessary to initiate recurrent cycles of thrombus formation and embolism when mice are placed under anesthesia with ket‐x and isoflurane, compared to pentobarbitone (n = 5‐6). (ii) Significantly fewer cycles per 10 min are seen in the isoflurane group. (C) (i) Area under the curve analysis shows that blood flow over an average of three cycles is correspondingly increased in isoflurane‐treated animals as validated in (ii) a representative blood flow chart from one animal per cohort (representative of n = 12 animals). Data is mean ± SEM. *P < 0.05; **P < 0.01; ***^* P < 0.001 (one‐way analysis of variance (ANOVA) with Tukey post‐hoc analysis)