| Antibodies |
|
|
| Rat Anti-SP |
Abcam |
Cat# Ab7340; RRID: AB_305866 |
| Chicken anti-GFP |
Life Technologies |
Cat# A10262; RRID: AB_2534023 |
| Alexa Fluor 488 |
Invitrogen |
Cat# A11039; RRID: AB_142924 |
| Alexa Fluor 547 |
Invitrogen |
Cat# A21247; RRID: AB_141778 |
| GS-IB4 Alexa 568 |
Invitrogen |
Cat# I21412
|
| rabbit anti-NF200 |
Chemicon |
Cat# AB1982; RRID: AB_2313731 |
| Anti-mouse CD16/32 Fc Blocker |
BioLegend |
Cat# 101320; RRID: AB_1574975 |
| CD45-FITC |
BioLegend |
Cat# 103108; RRID: AB_312973 |
| PerCP/Cy5.5 anti-mouse CD8a |
BioLegend |
Cat# 100733; RRID: AB_2075239 |
| Anti-mouse CD45 PE |
BioLegend |
Cat# 103105; RRID: AB_312970 |
| Anti-mouse CD11b BV510 |
BioLegend |
Cat# 101245; RRID: AB_2561390 |
| Anti-mouse c-Kit BV605 |
BioLegend |
Cat# 135121; RRID: AB_2562040 |
| Anti-mouse FcεRI FITC |
BioLegend |
Cat# 134305; RRID: AB_1626102 |
| Anti-mouse IgE FITC |
BioLegend |
Cat# 406905; RRID: AB_493288 |
| Chemicals, Peptides, and Recombinant Proteins |
|
|
| CFA |
Sigma |
Cat# F5881 |
| Substance P |
Tocris |
Cat# 1156 |
| SR 140333 |
Tocris |
Cat# 4012 |
| Anti-SP antibody for pain studies |
Millipore Sigma |
Cat# AB1566; RRID: AB_11213407 |
| Diphtheria Toxin |
Sigma |
Cat# D0564 |
| Isotype IgG |
Millipore Sigma |
Cat# NS01L |
| ZombieUV viability dye |
BioLegend |
Cat# 423107 |
| Human SCF |
Peprotech |
Cat# 300–07 |
| Avidin sulforhodamine |
Marker Gene Technologies |
Cat# M1124 |
| Goat Alexa Avidin |
Sigma |
Cat# 189727 |
| Critical Commercial Assays |
|
|
| Mouse CCL2 ELISA |
R&D Systems |
Cat# DY479 |
| Mouse CCL3 ELISA |
R&D Systems |
Cat# DY450 |
| Human TNF-alpha ELISA |
R&D Systems |
Cat# DY210 |
| Human CCL2 ELISA |
R&D Systems |
Cat# DY279 |
| Human CCL3 ELISA |
R&D Systems |
Cat# DY270 |
| Human CCL4 ELISA |
R&D Systems |
Cat# DY271 |
| Human GM-CSF ELISA |
R&D Systems |
Cat# DY215 |
| Human IL-8 ELISA |
R&D Systems |
Cat# DY208 |
| Human Inflammatory Cytokines Multi-Analyte ELISArray |
QIAGEN |
Cat# MEH-0004a |
| Experimental Models: Cell Lines |
|
|
| LAD2 |
NIH |
Metcalfe Lab |
| Experimental Models: Organisms/Strains |
|
|
| Mouse: Mrgprb2 Mut |
McNeil et al., 2015 |
N/A |
| Software and Algorithms |
|
|
| Prism 6.01 |
GraphPad Software |
N/A |
| FlowJo |
Tree Star |
N/A |
| Other |
|
|
| StemPro-34 SFM (1X) |
Thermo Fisher Scientific |
Cat# 10639011 |
| isoflurane Abbott Laboratories CAS# 26675–46–7 |
Abbott Laboratories |
Cas# 26675–46–7 |
| Lipofectamine 3000 |
Life Technologies |
Cat# L3000015 |
| SMARTpool: ON-TARGETplus MRGPRX2 siRNA |
Dharmacon |
Cat# L-005666–00–0005 |
| ON-TARGETplus Non-targeting Pool |
Dharmacon |
Cat# D-001810–10–05 |
