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. Author manuscript; available in PMC: 2019 Apr 15.
Published in final edited form as: Sci Immunol. 2018 Aug 24;3(26):eaat2738. doi: 10.1126/sciimmunol.aat2738

Fig. 1. NSA inhibits pyroptotic cell death in immortalized murine macrophages.

Fig. 1.

(A) Chemical structure of NSA. (B to E) Cells were primed with LPS, followed by NSA treatment 30 min before stimulation with nigericin (NG) or ATP. Pyroptotic pore formation was measured kinetically through the uptake of PI, and cell lysis was measured by LDH release. PI uptake is taken from four experiments each with technical duplicates. LDH release is from three independent experiments, each done in technical duplicate. *P < 0.05, **P < 0.01, and ***P < 0.001. (F) Measurement of pyroptotic pore formation assessed by PI uptake with simultaneous administration of NSA with nigericin stimulation. Data represent four experiments each with two technical replicates. (G) Bright-field and epifluorescent microscopy images of iBMDM cells with or without NSA treatment before and after treatment with 10 μM nigericin. Scale bars, 100 μm. All cells were primed with LPS (1 μg/ml) for 4 hours. (B to F) PI and LDH data show means ± SE. Tx, treatment.