Fig. 6.

MFSD12 is associated with poor prognosis in melanoma patients and required for tumor growth and progression in vivo. a Representative images of tumor tissues in different staining classifications are shown and graded from “−” to “+++”. b, c Kaplan–Meier curves showing the relationship between MFSD12 expression and overall and disease-free survival. Scale bar, 40×, 500 μm; 400×, 50 μm. d Subcutaneous xenograft tumor volumes were measured at the indicated number of days; representative pictures are shown. e, f All the tumors are shown at the 30th day, and the weights are presented in the bar graph. g Subcutaneous xenograft tumors stained with H&E and IHC. h, i Representative H&E-stained metastatic clusters are shown; the number of tumor metastatic clusters per lung are presented in the bar graph. ^*p < 0.05; ^**p < 0.01