Table 1. . Estimates of LOD and LOQ for cortisol metabolites in human urines.

Metabolites	LOD (pg/μl urine)	LOQ (pg/μl urine)
Cortisol	0.05	0.16
6-Hydroxycortisol	1.20	3.63
A-THF/THF	0.79	2.39
Dihydrocortisol	1.56	4.74
Cortisol sulfate	0.23	0.77
Cortisone	0.02	0.08
THE	1.72	5.21
Dihydrocortisone	5.81	17.61
Cortisone-sulfate	0.28	0.84

[²H₄]-cortisol, [²H₄]-6β-hydroxycortisol, [²H₆]-A-THF, [²H₈]-cortisone, [²H₆]-THE and [²H₄]-cortisol sulfate were employed as internal standards.

A-THF: Allo-3α-tetrahydrocortisol; THE: 5β-tetrahydrocortisone; THF: 5β-tetrahydrocortisol.