Table 2.
Levels of variables tested in Box Behnken Design (BBD) for optimization of protease production.
| Factor | Range and Levels | ||
|---|---|---|---|
| −1 | 0 | 1 | |
| X1 Temperature (°C) | 20 | 30 | 40 |
| X2 Glucose (g/L) | 8 | 11 | 14 |
| X3 pH | 4 | 6 | 8 |
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