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Box Behnken matrix and response results for optimization of protease.
| Run | Factors | Protease Activity U/mL | |||
|---|---|---|---|---|---|
| X1 (°C) | X2 (g/L) | X 3 | Experimental | Predicted | |
| 1 | −1 | −1 | 0 | 417.57 | 386.82 |
| 2 | 1 | −1 | 0 | 6.20 | 4.80 |
| 3 | −1 | 1 | 0 | 328.69 | 529.61 |
| 4 | 1 | 1 | 0 | 7.61 | 6.58 |
| 5 | −1 | 0 | −1 | 144.73 | 122.41 |
| 6 | 1 | 0 | −1 | 3.73 | 4.71 |
| 7 | −1 | 0 | 1 | 390.76 | 309.52 |
| 8 | 1 | 0 | 1 | 1.05 | 1.24 |
| 9 | 0 | −1 | −1 | 329.40 | 315.42 |
| 10 | 0 | 1 | −1 | 441.55 | 431.85 |
| 11 | 0 | −1 | 1 | 177.04 | 257.53 |
| 12 | 0 | 1 | 1 | 480.34 | 352.59 |
| 13 | 0 | 0 | 0 | 398.52 | 329.56 |
| 14 | 0 | 0 | 0 | 292.72 | 329.56 |
| 15 | 0 | 0 | 0 | 306.83 | 329.56 |
