Table 8.

Effect of metal ions (5 mM), detergents (0.1%) and enzyme inhibitors (5 mM) on protease activity of the endophytic fungus Penicillium bilaiae.

Chemicals	Concentrations	Units	Relative Activity (%)
Control	-	-	100
Cu²⁺	5	mM	21 ± 3
Ca²⁺	5	mM	32 ± 4
Fe²⁺	5	mM	54 ± 1
Zn²⁺	5	mM	100
Mn²⁺	5	mM	114 ± 2
Hg²⁺	5	mM	14 ± 4
B³⁺	5	mM	191 ± 1
Na²⁺	5	mM	100
PMSF	5	mM	0
EDTA	5	mM	100
β-Mercaptoethanol	5	mM	100
DTT	5	mM	100
Tween 80	0.1	%	181 ± 1
CHAPS	0.1	%	83 ± 1
Triton X-100	0.1	%	193 ± 2