Figure 2.

Mating assay and testing for pheromone production. Charcoal plate mating assay with wild type, Δptn1, Δptn1c, and FBD-tester strains. A positive mating reaction is indicated by a white “fuzz” phenotype of aerial hyphae production. The reduction in filamentation was noticeable at 24 h, while such differences disappeared by 48 h (not shown). Red boxes compare the ‘fuzz’ produced between wild type mating and Δptn1 mating. Green boxes indicate the ‘fuzz’ produced when Δptn1 strains were mixed with tester strains.