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. 2018 Dec 20;5(1):1. doi: 10.3390/jof5010001

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Response of mutants to cell wall stress. (A) 10-fold serial dilutions of the 1/2 a1b1, 2/9 a2b2, Δptn1 a1b1, Δptn1 a2b2, ptn1^otef a1b1, and ptn1^otef a2b2 strains were spotted onto media containing 1 mM Congo Red, and observations were made at 48 h. Congo Red is a cell wall stressor which prevents glycan microfibril assembly by binding to β-glucans. The 1/2 a1b1 strains were compared with a1b1 mutant strains, while a2b2 background strains were compared amongst themselves. Growth defects were observed in the Δptn1 strains, as well as ptn1^otef, as reductions in colony numbers at higher dilutions compared to comparable wild type, for both the a1b1 and a2b2 backgrounds. The overexpression of ptn1 ectopically in deletion mutants was able to partially rescue the growth defect in (B) Congo Red media and (C) calcofluor white media.

Response of mutants to cell wall stress. (A) 10-fold serial dilutions of the 1/2 a1b1, 2/9 a2b2, Δptn1 a1b1, Δptn1 a2b2, ptn1^otef a1b1, and ptn1^otef a2b2 strains were spotted onto media containing 1 mM Congo Red, and observations were made at 48 h. Congo Red is a cell wall stressor which prevents glycan microfibril assembly by binding to β-glucans. The 1/2 a1b1 strains were compared with a1b1 mutant strains, while a2b2 background strains were compared amongst themselves. Growth defects were observed in the Δptn1 strains, as well as ptn1^otef, as reductions in colony numbers at higher dilutions compared to comparable wild type, for both the a1b1 and a2b2 backgrounds. The overexpression of ptn1 ectopically in deletion mutants was able to partially rescue the growth defect in (B) Congo Red media and (C) calcofluor white media.