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. 2018 Dec 20;5(1):1. doi: 10.3390/jof5010001

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Chlorophyll estimation. (A) Chlorophyll count of leaves of plants infected with WT (1/2 a1b1 × 2/9 a2b2), Δptn1 (a1b1 × a2b2) and ptn1^otef (a1b1 × a2b2) were measured using the DMSO method [31]. (B) The plants infected with the Δptn1 strains had higher a/b ratios, and such plants had chlorophyll levels that were more comparable to “mock” infections. Means of chlorophyll a, chlorophyll b, and chlorophyll a/b ratio were marked with an asterisk (*) or two asterisks (**) were significantly different (p ≤ 0.05) and (p ≤ 0.01), respectively, by Tukey’s test.