Table 1.
Ustilago Strain | Genotype a | References |
---|---|---|
1/2 | a1b1 | [19] |
2/9 | a2b2 | [19] |
1/2 Δptn1 | a1b1 ptn1::cbxR | This study |
1/2 Δptn1J | a1b1 ptn1::hygR | This study |
2/9 Δptn1 | a2b2 ptn1::cbxR | This study |
2/9 Δptn1J | a2b2 ptn1::hygR | This study |
FB1 Δptn1J | a1b1 ptn1::hygR | This study |
1/2 ptn1otef | a1b1 Potef-ump2, cbxR | This study |
2/9 ptn1otef | a2b2 Potef-ump2, cbxR | This study |
SG200 | a1mfa2 bE1bW2 | [20] |
SG200 Δptn1 | a1mfa2 bE1bW2 ptn1::hygR | This study |
d132 | a1a2b1b2 | [21] |
d132 Δptn1 | a1a2b1b2 ptn1:: hygR | This study |
1/2 Δptn1c | a1b1 ptn1::cbxR Potef-ptn1, hygR | This study |
2/9 Δptn1c | a2b2 ptn1::cbxR Potef-ptn1, hygR | This study |
FBD11-7 | a1a1 b1b2 | [16] |
FBD12-17 | a2a2 b1b2 | [16] |
Plasmids | Genotype | References |
DelsGate ptn1 | kanR ptn1::cbxR | [22] |
Otef-ptn1-cbx | bla(ampR) Potef-ptn1 cbxR | This study |
Otef-ptn1-hyg | bla(ampR) Potef-ptn1 hygR | This study |
acbxR, carboxin resistance; hygR, hygromycin resistance; the otef promoter is a modified tef promoter in which two direct repeats of a synthetic fragment containing seven tetracycline-responsive elements precede the promoter.