Figure 4.
In vivo resolution assays of Salmonellae with precise chromosomal swaps of E. coli GyrA and GyrB subunits exploited supercoil reporters in six chromosome macrodomains. Macrodomain in Salmonella genome segments correspond to the six macrodomains of the E. coli chromosome: Ori-green; Right; Unstructured - black; Right, red; Ter, purple with black hatches showing matS sites; Left Domain, blue; Left unstructured domain, black. Resolution efficiencies at 10 positions are shown for: Salmonella WT, red; Strains with the E. coli GyrB transgene, black; Strains with E. coli GyrA, blue; and Salmonella with E. coli GyrA + GyrB, green.