Skip to main content
. 2019 Mar 15;7(3):81. doi: 10.3390/microorganisms7030081

Figure 6.

Figure 6

Resolution assays of WT E. coli and E. coli supercoiling with chimeric Salmonella–E. coli GyrA fusions. (A) A map of the genes that were tested. E. coli strains containing a WT GyrA, and two chimeric forms of Salmonella-E. coli GyrA were tested for supercoil-dependent resolution at four sites in the chromosome. (B) Resolvase deletion efficiencies for three gyrA genes are shown at 4 chromosomal locations. Strains with a WT GyrA and a Salmonella GyrA fused to the E. coli CTD at amino acid had very similar efficiencies at all four positions with a mean of 91 ± 5% and 92 ± 3% respectively. Strains carrying the Salmonella chimera fused to only the last 35 amino acids of the E. coli GyrA tail had a mean efficiency of 77 ± 4%, which shows a small (MIF) of 86.