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. 2019 Mar 25;8(3):411. doi: 10.3390/jcm8030411

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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mecA complex after ICG-PDT in MRSA/JD004, MRSA/BAA-1556, and MRSA/39452. Presence of mecA, mecI, mecR1, IS431, and IS1272 genes was examined after treatment. (A) After PDT, the mecA, mecI, and mecR1 genes were undetected in MRSA/JD004 except for IS431. (B,C) There are no changes in the mecA complex after treatment in MRSA/BAA-1556 and MRSA/39452. Lane 1: marker; lane 2: MSSA; lane 3: absolute control; lane 4: DC (dark control); lane 5: LC (light control); lane 6: PDT treatment. (D) Quantitative changes of mecA mRNA within three clinical MRSA isolates after treatment. mecA mRNA was analyzed by qRT-PCR. No mecA was detected in MSSA and MRSA/JD004 after PDT. mecA expression decreased about twofold in MRSA/BAA-1556 after PDT. No significant changes in mecA level in MRSA/39452. Results shown in (D) are the mean of three independent determinations ± standard deviation. *** p < 0.001, Bonferroni t method.

mecA complex after ICG-PDT in MRSA/JD004, MRSA/BAA-1556, and MRSA/39452. Presence of mecA, mecI, mecR1, IS431, and IS1272 genes was examined after treatment. (A) After PDT, the mecA, mecI, and mecR1 genes were undetected in MRSA/JD004 except for IS431. (B,C) There are no changes in the mecA complex after treatment in MRSA/BAA-1556 and MRSA/39452. Lane 1: marker; lane 2: MSSA; lane 3: absolute control; lane 4: DC (dark control); lane 5: LC (light control); lane 6: PDT treatment. (D) Quantitative changes of mecA mRNA within three clinical MRSA isolates after treatment. mecA mRNA was analyzed by qRT-PCR. No mecA was detected in MSSA and MRSA/JD004 after PDT. mecA expression decreased about twofold in MRSA/BAA-1556 after PDT. No significant changes in mecA level in MRSA/39452. Results shown in (D) are the mean of three independent determinations ± standard deviation. *** p < 0.001, Bonferroni t method.