Figure 1.

Generation of LPGAT1 knockout (KO) mice by CRISPR/Cas9 gene editing. (A) Strategy of guide RNA (gRNA) designing and LPGAT1 gene knockout. Two gRNAs were designed targeting exon 3 of the LPGAT1 gene, which resulted in a deletion of 124 bp nucleotides and termination of translation. (B) Genotyping of LPGAT1^-/- and WT control mice by PCR analysis. The homozygotes of LPGAT1 knockout showed 124-bp nucleotide deletion relative to the WT control mice. (C) RT-PCR analysis of LPGAT1 mRNA expression in livers of LPGAT1^-/- and the WT control mice. Data are represented as means ± SD. N = 3, ***P < .001 by t test. (D) Western blot analysis of LPGAT1 protein expression in the liver samples from LPGAT1^-/- and the WT control mice.