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. 2019 Mar 1;7(4):763–781. doi: 10.1016/j.jcmgh.2019.02.002

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© 2019 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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LPGAT1 deficiency caused hepatofibrosis. Male mice from Figure 2 were analyzed for (A and B) quantitative RT-PCR analysis of mRNA levels of (A) collagen I and (B) collagen III, and (C) Masson staining of fibrosis in liver sections from LPGAT1^-/- and WT controls. Fibrosis and dilation of hepatic venules are highlighted by arrows. Scale bar: 20 μm. (D) Quantification of fibrosis area in panel C by ImageJ software (1.47v; National Institutes of Health, Bethesda, MD). Data are represented as means ± SD (A and B, n = 4; and D, n = 6). **P < .01 by 1-way analysis of variance. KO, knockout; ND, normal chow diet.