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. 2019 Mar 1;7(4):763–781. doi: 10.1016/j.jcmgh.2019.02.002

Figure 7.

Figure 7

LPGAT1 deficiency aggravated lipid accumulation in primary hepatocytes. (A–C) Quantitative RT-PCR analysis of mRNA levels of genes that promote lipogenesis, including (A) PPARα, (B) SREBP1c, and (C) ACC1 in cultured primary hepatocytes isolated from WT and LPGAT1-/- mice. (D) Confocal imaging analysis of lipid droplets in cultured primary hepatocytes isolated from LPGAT1-/- and WT control mice in response to stimulation of oleic acids. Cells were incubated with oleic acids (200 μmol/L) for 16 hours. Lipid droplets were stained by BODIPY493/503, and highlighted by arrows. Scale bar: 10 μm. (E and F) Quantification of lipid droplet (E) number and (F) size in cultured primary hepatocytes stained with BODIPY493/503 by ImageJ (National Institutes of Health, Bethesda, MD). Data are representative of at least 3 independent experiments, and are represented as means ± SD (A–C, n = 5; E and F, n = 25–30 cells). *P < .05, **P < .01 by 1-way analysis of variance. CM, completed medium; Ctrl, control; KO, knockout; LD, lipid droplet; OA, oleic acid.