Figure 8.

LPGAT1 deficiency did not affect lipid accumulation in C2C12 myoblast cells. The LPGAT1 gene was deleted in C2C12 cells by CRISPR/Cas9 gene editing. The LPGAT1-deficient C2C12 cells and the vector controls were treated with vehicle or 200 μmol/L oleic acid for 16 hours, stained with Oil red O, and examined for lipid droplet biogenesis by microscope imaging analysis (All-in-One Fluorescence Microscope BZ-X800; Keyence, Osaka, Japan). Images are representative of 3 independent experiments. Scale bar: 50 μm. CM, completed medium; KO, knockout; OA, oleic acid.