Figure 2.

Both Paclitaxel Resistance and Oncolytic Vaccinia Virus Spread Are Recovered by the Overexpression of UCA1

(A) Enhanced UCA1 expression was confirmed by qRT-PCR (n = 3). (B) KF cell lines were treated with PTX (0, 2, 5, 20, 50, 100, 200, and 500 nM). Cell viability was determined 48 h after PTX treatment (n = 3). (C) EGFP (left) and bright-field (right) images of KF cell lines are shown after infection with OVV-LG (MOI = 0.01 and 0.001) for 72 h. Scale bar, 1,000 μm. (D) The intensity and area of viral EGFP brightness were measured using a Keyence BZ-X700 fluorescence microscope (n = 3). (E) Plaque-forming units (PFUs) of OVV were determined based on titration using RK-13 cells (n = 3). Data with error bars represent mean ± SEM.