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. 2019 Mar 26;13:35–48. doi: 10.1016/j.omto.2019.03.003

Figure 3.

Figure 3

Correlation between UCA1 and Oncolytic Vaccinia Virus Spread as Observed in Other Ovarian Cancer Cell Lines

(A and E) UCA1 expression was detected by qRT-PCR in SHIN-3, OVCAR3, RMG-1, SKOV3, and ES-2 cells (A) and primary ovarian cancer cells (E) (n = 3). (B and F) EGFP (left) and bright-field (right) images of SHIN-3, OVCAR3, RMG-1, SKOV3, and ES-2 cells (B) and primary ovarian cancer cells (F) after infection with OVV-LG (MOI = 0.001 or 0.01) for 48 h. Scale bar, 1,000 μm. (C and G) Intensity and area of viral EGFP brightness in SHIN-3, OVCAR3, RMG-1, SKOV3, and ES-2 cells (C) and primary ovarian cancer cells (G) were measured using a Keyence BZ-X700 fluorescence microscope (n = 3). (D and H) Plaque-forming units (PFUs) of OVV in SHIN-3, OVCAR3, RMG-1, SKOV3, and ES-2 cells (D) and primary ovarian cancer cells (H) were determined by titration using RK-13 cells (n = 3). Data with error bars represent mean ± SEM.