Figure 7.

UCA1 Expression Predicts the Therapeutic Effect of Paclitaxel and Oncolytic Vaccinia Virus-VGFΔ/O1LΔ

BALB/cAJcl-nu/nu mice were intraperitoneally injected with KFTX or KFlow cells stably expressing Renilla luciferase. After confirming tumor growth, PTX (1 mL, 10 mg/kg) in saline was intraperitoneally injected twice per week. Then 3 weeks later, mice were administered a single intraperitoneal injection of OVV-VGFΔ/O1LΔ (1 × 10⁶ PFUs) or PBS. (A) Tumor imaging is shown before and after PTX treatment (n = 14). (B) Bioluminescence signals (in photons/s) were calculated from the tumor imaging data. (C) Tumor imaging is shown after OVV-VGFΔ/O1LΔ injection (n = 7). (D) Bioluminescence signals (in photons/s) were calculated from the tumor imaging data. (E) Survival curves for the mice are shown after virus injection. A log-rank (Mantel-Cox) test was used to analyze significance. (F) At 3 weeks before and after PTX treatment, for the extraction of KFTX and KFlow tumors, mice were sacrificed, and tumors were collected and frozen quickly using liquid nitrogen. Collected tumors were disrupted in lysis buffer and UCA1 expression was detected by qRT-PCR (n = 9–15). ^∗∗p<0.01, ^∗∗∗p<0.001. Data with error bars represent mean ± SEM. Two-way ANOVA was used for (B) and (D).