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. 2019 Mar 13;38(8):e100312. doi: 10.15252/embj.2018100312

Figure 6. PIP5Kγ knockdown causes defects in autophagosome–lysosome fusion and tubulation of Rab7 compartments.

Figure 6

  • A, B
    Distribution of mCherry‐GFP‐LC3 in HEK293‐AT1 cells (without starvation) after treatment with control siRNA (A) or siRNA for PIP5Kγ (PIP5K1C). Scale bars: 20 μm. Note the massive tubulation of the LC3 compartment in some of the knock‐down cells (B, lower images, scale bar: 10 μm).
  • C
    Comparison of Pearson coefficients from cells treated with control or PIP5Kγ RNAi. For statistical analysis, the unpaired t‐test was used (n = 50 and 45 cells for control a PIP5Kγ knockdown groups, respectively; ***P < 0.0001).
  • D
    Representative confocal images of live HEK293‐AT1 cells expressing GFP‐Rab7 treated with control or PIP5Kγ RNAi. Note the tubulation in the PIP5Kγ RNAi‐treated cells. Scale bars: 10 μm.