Chemotaxis Characteristics of VV-HBD2-lacZ on DC Cells
(A) In vitro chemotaxis activity of VV-HBD2-lacZ or VV-lacZ on activated DCs was detected by transwell assay. The results are presented as mean ± SD of triplicate samples. The assay was repeated twice. (B) Increased number of pDCs (plasmacytoid dendritic cells) in melanoma tumor of mice vaccinated with VV-HBD2-lacZ. Tumor cells were stained with anti-mouse CD11c, CD45RA, and CD8. Gates were set on CD11+ cell population. The percentage of pDCs is presented as mean ± SD of three separate experiments (n = 3). *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. FITC, fluorescein isothiocyanate; APC, allophycocyanin.