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. 2019 Mar 8;19(4):2562–2567. doi: 10.1021/acs.nanolett.9b00319

Figure 2.

Figure 2

LDL particle induced transfer of amphiphilic cargo into nonsupported biomembranes. FCCS measurements on GUVs incubated with LDL particles. (a) Visualization of LDL interaction and cargo transfer into GUVs for LDL (scale bar = 10 μm). (b) Generalized polarization (GP) value determined with the polarity-sensitive dye C-Laurdan of GUVs alone and LDL blended GUVs represents lipid packing and its hydration level (N = 10). (c) Diffusion for C-BodipyFL and protein-Atto647N signal of fluorescently labeled LDL particles in solution and decorated GUVs, respectively. (d) FCS and FCCS on protein-Atto647N and C-BodipyFL. Shown are FCS and FCCS curves for the indicated probe molecules after fusion of LDL particles with GUV membranes (right) or in buffer solution (left). The left graph shows high cross-correlation when LDL particles were intact (magenta and orange curves virtually identical), whereas the graph on the right side shows no notable cross-correlation after LDL fusion with the DOPC membrane. Black lines represent the fits.