Gapdh	forward, 5′‐ ATGGTGAAGGTCGGTGTGAAC‐3′
	reverse, 5′‐ GCCTTGACTGTGCCGTTGAAT‐3′
Arx	forward, 5′‐ TCCGGATACCCCACTTAGCTT‐3′
	reverse, 5′‐ GACGCCCCTTTCCTTTAAGTG‐3′
Pax4	forward, 5′‐ ACCTCATCCCAGGCCTATCT‐3′
	reverse, 5′‐ AGGCCTCTTATGGCCAGTTT‐3′
Ngn3	forward, 5′‐ AGCTCTTGGCCCATAGATGATG‐3′
	reverse, 5′‐ AAGAAGGCAGATCACCTTCGTG‐3′
NeuroD1	forward, 5′‐ GCCCAGCTTAATGCCATCTTT‐3′
	reverse, 5′‐ CAAAAGGGCTGCCTTCTGTAA‐3′
Snai2	forward, 5′‐ TCTGCAGACCCACTCTGATG‐3′
	reverse, 5′‐ AGCAGCCAGACTCCTCATGT‐3′
Pax6	forward, 5′‐ AGTGAATGGGCGGAGTTATGA‐3′
	reverse, 5′‐ AACTTGGACGGGAACTGACA‐3′