Skip to main content
. Author manuscript; available in PMC: 2019 Apr 15.
Published in final edited form as: Methods Enzymol. 2018 Feb 26;601:391–418. doi: 10.1016/bs.mie.2017.11.037
Argument Synopsis (* = required) Detail
--t *Treatment BED file Type 1 ssDNA BED file
--c *Control BED file Type 1 ssDNA BED file
--gSz *Effective genome size Estimated size of mappable genome
--gName *Genome/species name Use pre-computed effective genome size
(can be used instead of --gSz) For human: hg19 | hg38 | hg | hs | human
For mouse: mm9 | mm10 | mm | mouse
For rat: rn | rat
--name *Name prefix for output files -
--out Output folder Default = current folder
--blist Blacklist file BED file of genomic regions with sequencing biases that result in spurious peak calls
i.e. for mouse (mm10 genome): $CHSPATH/mm10_hotspot_blacklist.bed
--tuniq Treatment BED file with ONLY unique fragments Optional, but not recommended
--cuniq Control BED file with ONLY unique fragments Optional, but not recommended
--debug DEBUG mode Builds scripts but does not execute.
Note: This is a logical argument, so does not take any value (pass as --debug)
--q30 Q30 mode Use only fragments where both reads have a q-score >= 30. This is useful for removing reads that map with low confidence (i.e. to high copy repeats).
Note: This is a logical argument, so does not take any value (pass as --q30)
--nc Do not call peaks Do not run peak calling.
Note: This is a logical argument, so does not take any value (pass as --debug)
--h/help Show help