Figure 3:

Absorbance and decadic attenuance (DA) measurements normalized to the percentage of reference values for a MV spectrophotometer (A) and a MV plate reader (B). The reference values for the NISTmAb were the values simple-corrected for absorbance at 340 nm and 320 nm (Table I) for the MV spectrophotometer and the MV plate reader measurements, respectively. Measurements were done on three separate days. The measurements were normalized to a 1-cm pathlength and then the values were calculated as the percentage of the reference values (Table I). For the NISTmAb measurements, the MV spectrophotometer and the reference values were corrected by subtracting the 340 nm values and the MV plate reader measurement, and reference values were corrected by subtracting the 320 nm values. The error bars are 1 coeficient of variation from n=3 measurements for the MV spectrophtometer and n=16 measurements for the MV plate reader.