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. 2019 Apr 12;15:1744806919841196. doi: 10.1177/1744806919841196

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© The Author(s) 2019

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 3. — Expression of five I_A-encoding Kv alpha-subunit mRNAs in a naïve rat DRG. DRG sections were processed for ISH histochemistry with ³⁵S-labeled RNA probes and visualized by emulsion autoradiography. Left panels: Representative dark-field photomicrographs of the DRG showing ISH signals for Kv1.4 (a), Kv3.4 (b), Kv4.1 (c), Kv4.2 (d), and Kv4.3 (e) mRNAs, respectively. Scale bar = 500 μm. Right panels: Scatterplot diagrams of the left-hand panels. Individual neuronal profiles are plotted according to the cross-sectional area (μm²; along the x-axis) and signal intensity (x background; along the y-axis). The lines at S/N ratio = 10 indicate the borderline between the negatively and positively labeled profiles.DRG: dorsal root ganglion; ISH: in situ hybridization; Kv: voltage-gated potassium channel; S/N: signal to noise.