Skip to main content
. Author manuscript; available in PMC: 2020 Apr 1.
Published in final edited form as: Biomaterials. 2019 Feb 5;200:15–24. doi: 10.1016/j.biomaterials.2019.02.004

Figure 7. Mesenchymal stem cells (MSCs) undergo enhanced osteogenic differentiation in alginate-PEG gels relative to alginate gels.

Figure 7.

A, Initial elastic modulus of stiff alginate-PEG gels for osteogenesis study. B, Stress relaxation tests for stiff alginate-PEG hydrogels. C, Quantification of relaxation time from stress relaxation tests. D, The expression of markers of osteogenesis including OCN, ALP, RUNX2 and BSP. E, Images of alkaline phosphatase staining (ALP, blue), indicating early osteogenic differentiation, for MSCs cultured in alginate-PEG gels for seven days. F, Quantification of ALP positive cells. Student t-tests; **, ***, and **** indicate p < 0.01, p < 0.001, and p < 0.0001, respectively. Scale bar is 50 µm.