KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| DUX4 | R&D Systems | Cat#MAB95351; RRID: AB_2754557 |
| Bacterial and Virus Strains | ||
| E. coli BL21(DE3) | Lucigen | Cat#60401–3 |
| E. coli 5α | New England BioLabs | Cat#C2987I |
| Stellar competent cells | Takara BIO INC. | Cat#636763 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| DMEM, high glucose | HyClone | Cat#SH30081.01 |
| FBS | PEAK serum | Cat#PS-FB3 |
| Penicillin/streptomycin | Life Technologies | Cat#15140–122 |
| Glutamax | Millipore | Cat#SCR006 |
| 0.25% Trypsin-EDTA | Life Technologies | Cat#25200–072 |
| PBS | HyClone | Cat#SH30256.01 |
| Doxycycline | Alfa Aesar | Cat#J60579–14 |
| XhoI | New England BioLabs | Cat#R0146S |
| HindIII-HF | New England BioLabs | Cat#R3104M |
| BsaI | New England BioLabs | Cat#R0535S |
| XbaI | New England BioLabs | Cat#R0145S |
| Recombinant Sumo protease (Ulp1 core) | This paper | N/A |
| LATaq Polymerase | Takara BIO INC. | Cat#RR02AG |
| In-Fusion HD Cloning Kit | Clontech | Cat#638909 |
| TransIT-LTI transfection reagent | Mirus Bio LLC | Cat#MIR2300 |
| QIAPrep Spin MiniPrep Kit | QIAGEN | Cat#27106 |
| Bis-Tris | Sigma | Cat#B9754–1KG |
| polyethylene glycol 3,350 | Hampton Research | Cat#HR2–591 |
| β-mercaptoethanol | Aldrich | Cat#M6250 |
| sodium chloride | Fisher | Cat#BP-358 |
| Triton X-100 | Acros | Cat#21568–2500 |
| Tris base | Fisher | Cat#BP152 |
| bovine serum albumin | Sigma | Cat#A7030 |
| bromophenol blue | Ricca Chemicals | Cat#1353–4 |
| glycerol | Fisher | Cat#S25342B |
| tris(2-carboxyethyl)phosphine-HCl | Biosynth | Cat#C-1818 |
| HisPur Ni-NTA resin | Thermo Scientific | Cat#88222 |
| Boric acid | Fisher | Cat#BP168–1 |
| EDTA | Fisher | Cat#BP120 |
| Imidazole | AK Scientific | Cat#D070 |
| Critical Commercial Assays | ||
| ONE-Glo Luciferase Assay System | Promega | Cat#E6120 |
| Deposited Data | ||
| Crystal structure data (atomic coordinates and structure factors) | This paper | PDB ID: 6E8C |
| Experimental Models: Cell Lines | ||
| iC2C12 | Bosnakovski et al., 2008b | N/A |
| Oligonucleotides | ||
| 5’-GCGTAATCTAATCAACA-3’ | Integrated DNA Technologies (IDT) | N/A |
| 5’-TGTTGATTAGATTACGC-3’ | IDT | N/A |
| /56-FAM/TGCGTAATCTAATCAACAC | IDT | N/A |
| GTGTTGATTAGATTACGCA | IDT | N/A |
| /56-FAM/TGCGTAATCTAATTAACAC | IDT | N/A |
| GTGTTAATTAGATTACGCA | IDT | N/A |
| /56-FAM/TGCGTGATCTAATCAACAC | IDT | N/A |
| GTGTTGATTAGATCACGCA | IDT | N/A |
| /56-FAM/TGCGTAATCTATCAACAC | IDT | N/A |
| GTGTTGATAGATTACGCA | IDT | N/A |
| /56-FAM/TT TCC CTT TTC CCC TTT TT | IDT | N/A |
| AAA AAG GGG AAA AGG GAA A | IDT | N/A |
| /56-FAM/AG CCC GCA CCA ACC ATG CC | IDT | N/A |
| GGC ATG GTT GGT GCG GGC T | IDT | N/A |
| /56-FAM/TG CGT AAT CTA GGG GAC AC | IDT | N/A |
| GTG TCC CCT AGA TTA CGC A | IDT | N/A |
| /56-FAM/TG CGC CCC CTA ATC AAC AC | IDT | N/A |
| GTG TTG ATT AGG GGG CGC A | IDT | N/A |
| Recombinant DNA | ||
| pE-SUMO-DUX4 (15–155) expression plasmid | This paper | N/A |
| p2lox | (Iacovino et al., 2011) | N/A |
| p2lox-DUX4 | (Bosnakovski et al., 2008b) | N/A |
| pGL4–2X-TAATCTAATCA | (Zhang et al., 2016b) | N/A |
| pGL4–2X-TAATCTAATTA | This paper | N/A |
| pGL4–2X-TGATTCAATCA | This paper | N/A |
| Software and Algorithms | ||
| XDS | (Kabsch, 2010) | N/A |
| PHASER | (McCoy et al., 2007) | N/A |
| COOT | (Emsley et al., 2010) | N/A |
| PHENIX | (Adams et al., 2010) | N/A |
| PyMOL | https://pymol.org/2/ | N/A |
| Prism (v6.07) | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |
| Other | ||
| 6% acrylamide native gel (0.5x TBE) | Invitrogen | EC63652BOX |
| Superdex 200 26/60 | GE | 17-1071-01 |