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. 2019 Apr 15;38:161. doi: 10.1186/s13046-019-1150-y

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Knockdown of LINC01354 inhibits tumor proliferation and metastasis in vivo. a Images of tumors formed from mice injected with shCtrl or shLINC01354 transfected LoVo cells. b-c The shLINC01354 transfection decreased the tumor size and weight in vivo. d IHC assays revealed that the Ki-67 and PCNA proliferation index decreased in tumors originated from mice injected with LINC01354 silenced LoVo cells in comparison to tumors from mice injected with shCtrl transfected LoVo cells. e The expressions of E-cadherin and N-cadherin were determined by IHC experiments. f The metastatic nodes in lung in tumors originated from mice injected with LINC01354 silenced LoVo cells and tumors from that with shCtrl transfected LoVo cells. Data were showed as the mean ± SD. All assays were carried out in triplicate, ^*p < 0.05, ^**p < 0.01 and ^***p < 0.001