Fig. 5.

Wnt/β-catenin pathway was involved in the function of LINC01354 in CRC cells. a 67 downregulated genes under LINC01354 silencing were found by mRNA profiling analysis. b-c It was found by GO and KEGG analysis that the Wnt/β-catenin signaling pathway involved in the downstream pathway of LINC01354. d-e qRT-PCR and western blot analysis of the expression of genes (CTNNB1, c-Myc, CycinD1, and MMP7) involved in Wnt/β-catenin signaling pathway in LoVo cells with silencing of LINC01354. f The inhibitive effect of LINC01354 downregulation on Wnt/β-catenin pathway was detected by TOP-Flash luciferase reporter assay. g CCK8 and EdU assays were conducted to measure the impact of DKK1 on the pro-proliferation capacity of HCT116 cells transfected with pcDNA3.1/LINC01354. h The promoting function of pcDNA3.1/LINC01354 on migration and invasion of HCT116 cells was abrogated when exposed to DKK1 as screened by transwell assays. Results were shown as the mean ± SD on the basis of 3 independent experiments, ^*p < 0.05, ^**p < 0.01