Fig. 6.

LINC01354 positively regulated β-catenin through modulating the CTNNB1 stability. a The mRNA level and protein level of β-catenin in HCT116 cells with overexpressing LINC01354 were measured by qRT-PCR and western blot. b The nucleus translocation induced by overexpression of LINC01354 was observed by IF assay. c A positive correlation between CTNNB1 and LINC01354 was tested by qRT-PCR. d Luciferase reporter assay demonstrated that the modulation of LINC01354 on CTNNB1 expression was not at transcriptional level. e Knockdown of LINC01354 decreased the stability of CTNNB1 with 7SL as negative control. f-g IHC and western blot results showed that silencing LINC01354 decreased the levels of c-Myc, β-catenin, and MMP-7 in vivo. Results were exhibited as the mean SD on the basis of three independent experiments, ^**p < 0.01 and R² = 0.080