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. 2019 Mar 6;294(15):6073–6081. doi: 10.1074/jbc.RA119.007925

Figure 1.

Figure 1.

hpol η can extend a DNA primer opposite an undamaged base or CPD by incorporation of dNTPs or rNTPs. hpol η (500 nm) and the annealed duplex (5 μm) were incubated with physiological concentrations of dNTPs (dATP, 25 μm; dCTP, 30 μm; dGTP, 90 μm; and dTTP, 40 μm) or rNTPs (ATP, 2 mm; CTP, 0.25 mm; GTP, 0.5 mm; and UTP, 0.5 mm) (44) for 5, 10, 30, and 60 min. A, DNA/DNA (dT). B, DNA/DNA (CPD). dATP and rATP competed for incorporation by hpol η (100 nm) in the presence of different concentrations of dATP, rATP, or mixtures of the two. Incubations were done for 5 min. C and D, the annealed substrates were (C) DNA/DNA (dT) (1 μm) and (D) DNA/DNA (CPD) (1 μm). See “Oligonucleotide substrates” under “Experimental procedures” for the oligonucleotide sequences used.