Figure 2.

Effect of Ca²⁺ and pharmacological inhibitors on activity of BdALMT12 after malate activation. Statistical tests and notations are described under “Experimental procedures,” see “Electrophysiology.” A, Ca²⁺-dependent Δ current density of BdALMT12. HEK cells transfected with BdQUAC1 were patched using different Ca²⁺ concentrations in the pipette solution including 0 μm (black circle), 0.05 μm (white circle), 0.1 μm (black triangle), 0.5 μm (white triangle), and 5 μm (black square) free Ca²⁺, n ≥ 8 for each concentration. B, conductance of BdALMT12 channels at different Ca²⁺ concentrations, including 0.1 μm (solid line), 0.5 μm (broken line), and 5 μm (dotted line) free Ca²⁺. No statistical differences were detected (p > 0.05). C, representative traces of BdALMT12 current, presented in current density (pA/pF), at −180 mV with 0 and 5 μm free Ca²⁺ in the pipette solution. D, effect of staurosporine. Pipette solutions contained 0.1 μm free Ca²⁺ (white circle) or 0.1 μm free Ca²⁺ and 60 nm staurosporine (black circle) with n = 9 for each treatment. E, conductance of BdQUAC1 channels with addition of staurosporine in the pipette solution. Pipette solutions contained 0.1 μm free Ca²⁺ (solid line) or 60 nm staurosporine (dotted line). F, representative traces of BdQUAC1 current, presented in current density (pA/pF), at −180 mV with 0.5 μm free Ca²⁺ and the presence or absence of W-7. G, effect of W-7. Pipette solutions contained 0.5 μm free Ca²⁺ (black triangle), 0.5 μm free Ca²⁺ and 1 μm W-7 (white triangle), 0.5 μm free Ca²⁺ and 5 μm W-7 (black circle), 0.5 μm free Ca²⁺ and 10 μm W-7 (black circle), and 0 μm free Ca²⁺ (black square) with n ≥ 6 for each treatment. H, conductance of BdQUAC1 channels with addition of W-7 in the pipette solution. Pipette solutions contained 0.5 μm free Ca²⁺ (broken line), 0.5 μm free Ca²⁺ and 1 μm W-7 (dotted line), or 0.5 μm free Ca²⁺ and 5 μm W-7 (solid line). I, voltage protocol used for the experiments.