Table 2.

Binding parameters of wildtype and variant CaM-binding peptide

Unless otherwise indicated, all measurements were made in the presence of 5 mm Ca²⁺. The “no Ca²⁺” condition included 1.5 mm EGTA. W-7 concentration was equimolar to CaM concentration. ND means not determined.

Peptide	Kd1	ΔH1	ΔS1	Kd2	ΔH2	ΔS2
	m	kJ/mol	J/mol·K	m	kJ/mol	J/mol·K
Wildtype peptide	3.452E−8	105.4	4.962E2	3.925E−6	−174.0	−4.8E2
Wildtype peptide, no Ca2+	9.165E−7	69.16	3.437E2	1.003E−5	−199.9	−5.636E2
Variant 338/342 peptide	4.101E−8	−48.78	−2.217E1	6.366E−6	−199.2	−5.685E2
Variant 335/338 peptide	3.721E−7	−75.77	−131.1	ND	ND	ND
Variant 335/342 peptide	5.5E−6	−470.8	−1478	ND	ND	ND
Variant 335/338/342 peptide	ND	ND	ND	ND	ND	ND
Wildtype peptide + W-7	ND	ND	ND	ND	ND	ND