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. 2019 Feb 15;294(15):6142–6156. doi: 10.1074/jbc.RA118.005301

Table 4.

Primer sequences

F is forward, and R is reverse.

Name Primer sequence
F-P1 5′-CACCGCCGACAAGTTTGTACAAAAAAGCAGGCTTAATAGCTAGCGAGGCCACCATGGCTTGCACTCTACATTCC-3′
R-P1 5′-GCCGACCACTTTGTACAAGAAAGCTGGGTACCGCGGTTATTATTCAGCTGCAGTAGAAACTGT-3′
F-R338A 5′-CCATGCACTAGAGTAGCAGCGGAAGTGGCCAAGGTTTGC-3′
R-R338A 5′-GCAAACCTTGGCCACTTCCGCTGCTACTCTAGTGCATGG-3′
F-DM1 5′-CGAAATCCATGCACTGCAGTAGCAGCGGAAGTGGC-3′,
R-DM1 5′-GCCACTTCCGCTGCTACTGCAGTGCATGGATTTCG-3′
F-TM 5′-CGAAATCCATGCACTGCAGTAGCAGCGGAAGTGGCCGCGGTTCTACAAGAGCT-3′
R-TM 5′-AGCTCTTGTAGAACCGCGGCCACTTCCGCTGCTACTGCAGTGCATGGATTTCG-3′
F-DM2 5′-CCATGCACTGCAGTAGCACGAGAAGTGGCCGCGGTTCTA-3′
R-DM2 5′-TAGAACCGCGGCCACTTCTCGTGCTACTGCAGTGCATGG-3′
F-DM3 5′-CGAAATCCATGCACTAGAGTAGCAGCGGAAGTGGCC-3′
R-DM3 5′-GGCCACTTCCGCTGCTACTCTAGTGCATGGATTTCG-3′
F-BdUBC18 5′-GGAGGCACCTCAGGTCATTT-3′
R-BdUBC18 5′-ATAGCGGTCATTGTCTTGCG-3′
F-BdALMT12 5′-ACTGTTGCTGCACTTCATGG-3′
R-BdALMT12 5′-CATTGCGTTCTGGTGGTTGA-3′
F-myc 5′-GATATACCGCGGGCCCGGGATCCGCCCCTCTCC-3′
R-myc 5′-GATATACCGCGGTTATAGGTCCTCTTCGCTGATTAGCTTTTGTTCTTCAGCTGCAGTAGAAACTGTGTGGCTGCC-3′